We reviewed studies of alcohol-regulated epigenetic changes in neural stem cells as a model of fetal alcohol syndrome. Our focus was on the technology used to assess changes in DNA methylation, including the analysis of data. Based on advances in methods, we recommend using whole-genome or sequence-selected genome sequencing using bisulfite conversion. Data analysis methods have evolved but mainly focus on comparing differentially methylated regions.
The article appeared in the October 2016 issue of Current Pharmacology Reports. For some reason it has not yet made it into PubMed.
njstem::hart_lab@rutgers 