Today our new paper on nicotine addiction appeared in Scientific Reports. We found that excitatory neurons made from subjects carrying the rs16969968 SNP variant in the CHRNA5 gene (encoding nAChR α5 subunit) exhibited an increased response to lower doses of nicotine that rapidly desensitized as the concentration of nicotine was increased. We used induced pluripotent stem cells made from human lymphocytes stored in a genetics repository. Our results are consistent with the idea that people carrying this risk variant of CHRNA5 (called N398) may respond more strongly to initial exposure to nicotine but then may have a harder time reaching this same response with subsequent doses.
The initial version on Scientific Report’s website used an old version of the Supplemental Materials. Here is the updated version, including active links to supporting Supplemental Table 3 and Supplemental Table 4.
RNAseq data for this publication are available on GEO under accession number GSE56398.