Modeling nicotine addiction with stem cells

Today our new paper on nicotine addiction appeared in Scientific Reports.  We found that excitatory neurons made from subjects carrying the rs16969968 SNP variant in the CHRNA5 gene (encoding nAChR α5 subunit) exhibited an increased response to lower doses of nicotine that rapidly desensitized as the concentration of nicotine was increased.  We used induced pluripotent stem cells made from human lymphocytes stored in a genetics repository.  Our results are consistent with the idea that people carrying this risk variant of CHRNA5 (called N398) may respond more strongly to initial exposure to nicotine but then may have a harder time reaching this same response with subsequent doses.

The initial version on Scientific Report’s website used an old version of the Supplemental Materials.  Here is the updated version, including active links to supporting Supplemental Table 3 and Supplemental Table 4.

RNAseq data for this publication are available on GEO under accession number GSE56398.


Leave a Reply

Fill in your details below or click an icon to log in: Logo

You are commenting using your account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

This site uses Akismet to reduce spam. Learn how your comment data is processed.